Biotin-conjugated APOA4 Monoclonal antibody

APOA4 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IHC

Conjugate

Biotin

CloneNo.

3A9A8

Cat no : Biotin-66252

Synonyms

APOA4, apolipoprotein A IV

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Validation Data Gallery

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  IHC staining of mouse small intestine using Biotin-66252

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using Biotin-66252 (APOA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-66252 targets APOA4 in IHC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: APOA4 fusion protein Ag12454
• Full Name: apolipoprotein A-IV
• Calculated Molecular Weight: 396 aa, 45 kDa
• Observed Molecular Weight: 45-46 kDa
• GenBank Accession Number: BC074764
• Gene Symbol: APOA4
• Gene ID (NCBI): 337
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote INS secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.