APOA4 Monoclonal antibody

APOA4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3A9A8

Cat no : 66252-1-Ig

Synonyms

APOA4, apolipoprotein A IV

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Validation Data Gallery

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  WB analysis of human plasma using 66252-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66252-1-Ig (APOA4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 66252-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66252-1-Ig (APOA4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse small intestine using 66252-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66252-1-Ig (APOA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 66252-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66252-1-Ig (APOA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 66252-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using APOA4 antibody (66252-1-Ig, Clone: 3A9A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

• Positive WB detected in: human plasma tissue, L02 cells
• Positive IHC detected in: mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66252-1-Ig targets APOA4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: APOA4 fusion protein Ag12454
• Full Name: apolipoprotein A-IV
• Calculated Molecular Weight: 396 aa, 45 kDa
• Observed Molecular Weight: 45-46 kDa
• GenBank Accession Number: BC074764
• Gene Symbol: APOA4
• Gene ID (NCBI): 337
• RRID: AB_2881640
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote INS secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.