APOA4 Monoclonal antibody, PBS Only

APOA4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A9A8

Cat no : 66252-1-PBS

Synonyms

APOA4, apolipoprotein A IV

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Validation Data Gallery

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  WB analysis of human plasma using 66252-1-Ig (same clone as 66252-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66252-1-Ig (APOA4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66252-1-Ig (same clone as 66252-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66252-1-Ig (APOA4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 66252-1-Ig (same clone as 66252-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66252-1-Ig (APOA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 66252-1-Ig (same clone as 66252-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66252-1-Ig (APOA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 66252-1-Ig (same clone as 66252-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using APOA4 antibody (66252-1-Ig, Clone: 3A9A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

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Product Information

66252-1-PBS targets APOA4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: APOA4 fusion protein Ag12454
• Full Name: apolipoprotein A-IV
• Calculated Molecular Weight: 396 aa, 45 kDa
• Observed Molecular Weight: 45-46 kDa
• GenBank Accession Number: BC074764
• Gene Symbol: APOA4
• Gene ID (NCBI): 337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote INS secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.