APC2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

APC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13559-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 13559-1-AP (same clone as 13559-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13559-1-AP (APC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13559-1-PBS in a different storage buffer formulation.

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  WB analysis using 13559-1-AP (same clone as 13559-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13559-1-AP (APC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13559-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 13559-1-AP (same clone as 13559-1-PBS)

  IP result of anti-APC2 (IP:13559-1-AP, 4ug; Detection:13559-1-AP 1:300) with MCF-7 cells lysate 3200ug. This data was developed using the same antibody clone with 13559-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13559-1-AP (same clone as 13559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13559-1-AP (APC2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13559-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13559-1-AP (same clone as 13559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13559-1-AP (APC2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13559-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13559-1-AP (same clone as 13559-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 13559-1-AP (APC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13559-1-PBS in a different storage buffer formulation.

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Product Information

13559-1-PBS targets APC2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APC2 fusion protein Ag4427
• Full Name: anaphase promoting complex subunit 2
• Calculated Molecular Weight: 822 aa, 94 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC032503
• Gene Symbol: APC2
• Gene ID (NCBI): 29882
• RRID: AB_2227055
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.