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  • Featured Product
  • KD/KO Validated

APC2 Polyclonal antibody

APC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13559-1-AP

Synonyms

Anaphase-promoting complex subunit 2, ANAPC2, ANAPC 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APC2 Polyclonal antibody (13559-1-AP)

    WB analysis using 13559-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13559-1-AP (APC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using APC2 Polyclonal antibody (13559-1-AP)

    WB analysis using 13559-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13559-1-AP (APC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using APC2 Polyclonal antibody (13559-1-AP)

    IP experiment of MCF-7 using 13559-1-AP

    IP result of anti-APC2 (IP:13559-1-AP, 4ug; Detection:13559-1-AP 1:300) with MCF-7 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APC2 Polyclonal antibody (13559-1-AP)

    IHC staining of human breast cancer using 13559-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13559-1-AP (APC2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APC2 Polyclonal antibody (13559-1-AP)

    IHC staining of human breast cancer using 13559-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13559-1-AP (APC2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using APC2 Polyclonal antibody (13559-1-AP)

    IF Staining of MCF-7 using 13559-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 13559-1-AP (APC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13559-1-AP targets APC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APC2 fusion protein Ag4427
Full Name anaphase promoting complex subunit 2
Calculated Molecular Weight 822 aa, 94 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC032503
Gene Symbol APC2
Gene ID (NCBI) 29882
RRIDAB_2227055
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for APC2 antibody 13559-1-APDownload protocol
IHC protocol for APC2 antibody 13559-1-APDownload protocol
IF protocol for APC2 antibody 13559-1-APDownload protocol
IP protocol for APC2 antibody 13559-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

FASEB J

APC2CDH1 negatively regulates agrin signaling by promoting the ubiquitination and proteolytic degradation of DOK7.

Authors - Aizhong Chen
  • KD Validated
View Article
humanWB

FEBS J

The MAD2B-APC/C-MDM2 axis mediates acute kidney injury by modulating p53

Authors - Zhe Hao
View Article
mouseIF

Nat Commun

CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I

Authors - Cheng-Jie Zhou
View Article
humanWB

Adv Sci (Weinh)

CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC

Authors - Qi Li
View Article