CoraLite® Plus 488-conjugated AMZ2 Polyclonal antibody

AMZ2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16664

Synonyms

EC:3.4.-.-, BM-014, Archeobacterial metalloproteinase-like protein 2, Archaemetzincin-2, Archaemetzincin 2

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-16664

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 AMZ2 antibody (CL488-16664) at dilution of 1:200, CL594-Phalloidin (red).

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  IF Staining of HepG2 using CL488-16664

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 AMZ2 antibody (CL488-16664) at dilution of 1:200.

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  FC experiment of HepG2 using CL488-16664

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated AMZ2 Polyclonal antibody (CL488-16664)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-16664 targets AMZ2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMZ2 fusion protein Ag10141
• Full Name: archaelysin family metallopeptidase 2
• Calculated Molecular Weight: 360 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC056271
• Gene Symbol: AMZ2
• Gene ID (NCBI): 51321
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

AMZ2 (archaelysin family metallopeptidase 2) is a zinc metalloprotease. Its calculated molecular weight and observed molecular weight are both 41kDa.