AMPK Gamma 2 Polyclonal antibody, PBS Only

AMPK Gamma 2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12568-1-PBS

Synonyms

AMPK gamma2, PRKAG2, AMPK subunit gamma-2, AMPK subunit gamma 2, AAKG2

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Validation Data Gallery

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  WB analysis using 12568-1-AP (same clone as 12568-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 12568-1-AP (same clone as 12568-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse eye using 12568-1-AP (same clone as 12568-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 12568-1-AP (same clone as 12568-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12568-1-AP (same clone as 12568-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 12568-1-AP (same clone as 12568-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 12568-1-AP (same clone as 12568-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 12568-1-AP (same clone as 12568-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12568-1-AP (same clone as 12568-1-PBS)

  Immunofluorescent analysis of HeLa cells using 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

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Product Information

12568-1-PBS targets AMPK Gamma 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMPK Gamma 2 fusion protein Ag3214
• Full Name: protein kinase, AMP-activated, gamma 2 non-catalytic subunit
• Calculated Molecular Weight: 569 aa, 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC020540
• Gene Symbol: PRKAG2
• Gene ID (NCBI): 51422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma2 is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart.