AMPK Gamma 2 Polyclonal antibody

AMPK Gamma 2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF

Conjugate

Unconjugated

Publications

2

Cat no : 12568-1-AP

Synonyms

AAKG, AAKG2, AMPK gamma2, AMPK subunit gamma 2, CMH6, H91620p, PRKAG2, WPWS

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Validation Data Gallery

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  WB analysis of mouse heart using 12568-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of PC-3 using 12568-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12568-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 12568-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12568-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 12568-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12568-1-AP (AMPK gamma2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12568-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12568-1-AP (AMPK gamma2 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 12568-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12568-1-AP (AMPK gamma2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 12568-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12568-1-AP (AMPK gamma2 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 12568-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12568-1-AP (AMPK gamma2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 12568-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12568-1-AP (AMPK gamma2 antibody at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 12568-1-AP

  Immunofluorescent analysis of HeLa cells using 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, HEK-293 cells, HeLa cells, mouse eye tissue, PC-3 cells
• Positive IHC detected in: human heart tissue, human ovary tumor tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

12568-1-AP targets AMPK Gamma 2 in WB, IHC, IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMPK Gamma 2 fusion protein Ag3214
• Full Name: protein kinase, AMP-activated, gamma 2 non-catalytic subunit
• Calculated molecular weight: 569 aa, 63 kDa
• Observed molecular weight: 63 kDa
• GenBank accession number: BC020540
• Gene symbol: PRKAG2
• Gene ID (NCBI): 51422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma2 is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart.

Publications

Species	Application	Title
human	WB	J Med Chem; Identification of Pyruvate Carboxylase as the Cellular Target of Natural Bibenzyls with Potent Anticancer Activity against Hepatocellular Carcinoma via Metabolic Reprogramming.; Authors - Yuwen Sheng
human	WB,IF	J Cardiol; Identification and functional analysis of a novel PRKAG2 mutation responsible for Chinese PRKAG2 cardiac syndrome reveal an important role of non-CBS domains in regulating the AMPK pathway.; Authors - Zhang Bi-Li BL