AMPK Gamma 2 Polyclonal antibody

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  WB analysis using 12568-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12568-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12568-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye using 12568-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12568-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12568-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 12568-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 12568-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12568-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AMPK Gamma 2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12568-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

AMPK gamma2, PRKAG2, 5'-AMP-activated protein kinase subunit gamma-2, AAKG, AAKG2

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse eye tissue, PC-3 cells, Neuro-2a cells, mouse heart tissues, rat heart tissues
• Positive IHC detected in: human stomach tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

12568-1-AP targets AMPK Gamma 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3214

  Product name: Recombinant human PRKAG2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 11-341 aa of BC020540

  Sequence: EAGMLEKLEFEDEAVEDSESGVYMRFMRSHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWESKKQSFVGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRELYLQETFKPLVNISPDASLFDAVYSLIKNKIHRLPVIDPISGNALYILTHKRILKFLQLFMSDMPKPAFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKCNKLEILETIVDRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPAGAKQKETETE

• Full Name: protein kinase, AMP-activated, gamma 2 non-catalytic subunit
• Calculated Molecular Weight: 569 aa, 63 kDa
• Observed Molecular Weight: 63-70 kDa, 38 kDa
• GenBank Accession Number: BC020540
• Gene Symbol: PRKAG2
• Gene ID (NCBI): 51422
• RRID: AB_2252960
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UGJ0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma2 is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Med Chem; Identification of Pyruvate Carboxylase as the Cellular Target of Natural Bibenzyls with Potent Anticancer Activity against Hepatocellular Carcinoma via Metabolic Reprogramming.; Authors - Yuwen Sheng
human	WB,IF	J Cardiol; Identification and functional analysis of a novel PRKAG2 mutation responsible for Chinese PRKAG2 cardiac syndrome reveal an important role of non-CBS domains in regulating the AMPK pathway.; Authors - Zhang Bi-Li BL