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AMPK Gamma 2 Polyclonal antibody

AMPK Gamma 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12568-1-AP

Synonyms

AMPK gamma2, PRKAG2, AMPK subunit gamma-2, AMPK subunit gamma 2, AAKG2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    WB analysis using 12568-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    WB analysis of HeLa using 12568-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    WB analysis of PC-3 using 12568-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    WB analysis of mouse eye using 12568-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    WB analysis of HEK-293 using 12568-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 12568-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 12568-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    IHC staining of human stomach using 12568-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    IF Staining of HeLa using 12568-1-AP

    Immunofluorescent analysis of HeLa cells using 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, PC-3 cells, mouse eye tissue, HeLa cells, Neuro-2a cells, mouse heart tissues, rat heart tissues
Positive IHC detected inhuman stomach tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12568-1-AP targets AMPK Gamma 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMPK Gamma 2 fusion protein Ag3214
Full Name protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Calculated Molecular Weight 569 aa, 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC020540
Gene Symbol PRKAG2
Gene ID (NCBI) 51422
RRIDAB_2252960
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma2 is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart.

Protocols

Product Specific Protocols
WB protocol for AMPK Gamma 2 antibody 12568-1-APDownload protocol
IHC protocol for AMPK Gamma 2 antibody 12568-1-APDownload protocol
IF protocol for AMPK Gamma 2 antibody 12568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Med Chem

Identification of Pyruvate Carboxylase as the Cellular Target of Natural Bibenzyls with Potent Anticancer Activity against Hepatocellular Carcinoma via Metabolic Reprogramming.

Authors - Yuwen Sheng
View Article
humanWB,IF

J Cardiol

Identification and functional analysis of a novel PRKAG2 mutation responsible for Chinese PRKAG2 cardiac syndrome reveal an important role of non-CBS domains in regulating the AMPK pathway.

Authors - Zhang Bi-Li BL
View Article