AMPD2 Monoclonal antibody, PBS Only

AMPD2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C4C1

Cat no : 67430-1-PBS

Synonyms

AMP deaminase 2, AMP deaminase isoform L, AMPD2

---

Validation Data Gallery

• 

  WB analysis using 67430-1-Ig (same clone as 67430-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67430-1-Ig (same clone as 67430-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67430-1-Ig (same clone as 67430-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 67430-1-Ig (same clone as 67430-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67430-1-Ig (same clone as 67430-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 67430-1-Ig (same clone as 67430-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67430-1-Ig (same clone as 67430-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67430-1-Ig (AMPD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67430-1-Ig (same clone as 67430-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67430-1-Ig (AMPD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67430-1-Ig (same clone as 67430-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMPD2 antibody (67430-1-Ig, Clone: 2C4C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

---

Product Information

67430-1-PBS targets AMPD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: AMPD2 fusion protein Ag8433
• Full Name: adenosine monophosphate deaminase 2 (isoform L)
• Calculated Molecular Weight: 879aa,101 kDa; 798aa,92 kDa
• Observed Molecular Weight: 101 kDa
• GenBank Accession Number: BC007711
• Gene Symbol: AMPD2
• Gene ID (NCBI): 271
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Adenosine monophosphate deaminase (AMPD) catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle. Three AMPDs are present in mammals named AMPD1/2/3. AMPD2 is the main activity present in adult human liver and is widely expressed in non-muscle tissues and cells (PMID: 8526848). AMPD2 has some isoforms with the molecular mass of 88-101 kDa.