AMPD2 Monoclonal antibody

AMPD2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C4C1

Cat no : 67430-1-Ig

Synonyms

AMP deaminase 2, AMP deaminase isoform L, AMPD2



Tested Applications

Positive WB detected inHSC-T6 cells, 4T1 cells, HeLa cells, HepG2 cells, L02 cells, Neuro-2a cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67430-1-Ig targets AMPD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen AMPD2 fusion protein Ag8433
Full Name adenosine monophosphate deaminase 2 (isoform L)
Calculated Molecular Weight879aa,101 kDa; 798aa,92 kDa
Observed Molecular Weight 101 kDa
GenBank Accession NumberBC007711
Gene Symbol AMPD2
Gene ID (NCBI) 271
RRIDAB_2882668
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adenosine monophosphate deaminase (AMPD) catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle. Three AMPDs are present in mammals named AMPD1/2/3. AMPD2 is the main activity present in adult human liver and is widely expressed in non-muscle tissues and cells (PMID: 8526848). AMPD2 has some isoforms with the molecular mass of 88-101 kDa.

Protocols

Product Specific Protocols
WB protocol for AMPD2 antibody 67430-1-IgDownload protocol
IHC protocol for AMPD2 antibody 67430-1-IgDownload protocol
IF protocol for AMPD2 antibody 67430-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols