CoraLite® Plus 488-conjugated ALDOB Monoclonal antibody

ALDOB Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, rabbit

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3D2E4

Cat no : CL488-68396

Synonyms

Liver-type aldolase, Fructose-bisphosphate aldolase B, EC:4.1.2.13, ALDB, 3D2E4

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-68396

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ALDOB antibody (CL488-68396, Clone: 3D2E4 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68396 targets ALDOB in IF/ICC applications and shows reactivity with human, mouse, rat, rabbit samples.

• Tested Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ALDOB fusion protein Ag12823
• Full Name: aldolase B, fructose-bisphosphate
• Calculated Molecular Weight: 316 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC029399
• Gene Symbol: ALDOB
• Gene ID (NCBI): 229
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A (ALDOA), B (ALDOB), and C (ALDOC). Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183). This antibody may detect ALDOA.