ALDOB Monoclonal antibody

ALDOB Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3D2E4

Cat no : 68396-1-Ig

Synonyms

ALDB, ALDOB, Liver type aldolase

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Validation Data Gallery

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  WB analysis using 68396-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68396-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68396-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human urine using 68396-1-Ig

  human urine sample were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 68396-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ALDOB antibody (68396-1-Ig, Clone: 3D2E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: rat kidney tissue, human urine sample, rabbit liver tissue, rat liver tissue, mouse liver tissue, mouse kidney tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68396-1-Ig targets ALDOB in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

• Tested Reactivity: Human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ALDOB fusion protein Ag12823
• Full Name: aldolase B, fructose-bisphosphate
• Calculated Molecular Weight: 316 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC029399
• Gene Symbol: ALDOB
• Gene ID (NCBI): 229
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A (ALDOA), B (ALDOB), and C (ALDOC). Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183). This antibody may detect ALDOA.