CoraLite® Plus 488-conjugated AIRE Monoclonal antibody
AIRE Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, pig
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1A3C3
Cat no : CL488-66262
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66262 targets AIRE in IF/ICC, FC (Intra) applications and shows reactivity with human, pig samples.
Tested Reactivity | human, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | AIRE fusion protein Ag19103 |
Full Name | autoimmune regulator |
Calculated Molecular Weight | 545 aa, 58 kDa |
Observed Molecular Weight | 58-69 kDa |
GenBank Accession Number | BC137270 |
Gene Symbol | AIRE |
Gene ID (NCBI) | 326 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 AIRE antibody CL488-66262 | Download protocol |
FC protocol for CL Plus 488 AIRE antibody CL488-66262 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |