AIRE Monoclonal antibody

AIRE Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66262-1-Ig
Clone No.1A3C3

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF/ICC, ELISA

PGA1, APSI, APS1, APECED, AIRE1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inhuman spleen tissue, pig spleen tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66262-1-Ig targets AIRE in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AIRE fusion protein Ag19103 Predict reactive species
Full Name autoimmune regulator
Calculated Molecular Weight 545 aa, 58 kDa
Observed Molecular Weight 58-69 kDa
GenBank Accession NumberBC137270
Gene Symbol AIRE
Gene ID (NCBI) 326
RRIDAB_2881649
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43918
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AIRE antibody 66262-1-IgDownload protocol
IF protocol for AIRE antibody 66262-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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