CoraLite® Plus 647-conjugated AIF Monoclonal antibody

AIF Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

8C12B2

Cat no : CL647-67791

Synonyms

AIF, AIFM1, Mitochondrion Marker, PDCD8

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Validation Data Gallery

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  IF Staining of human kidney using CL647-67791

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 647 AIF antibody (CL647-67791, Clone: 8C12B2 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human kidney tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67791 targets AIF in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: AIF fusion protein Ag12400
• Full Name: apoptosis-inducing factor, mitochondrion-associated, 1
• Calculated Molecular Weight: 609 aa, 66 kDa
• Observed Molecular Weight: 67 kDa, 57 kDa
• GenBank Accession Number: BC111065
• Gene Symbol: AIFM1
• Gene ID (NCBI): 9131
• RRID: AB_2920311
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).