AIF Monoclonal antibody

• Featured Product
• KD/KO Validated

AIF Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, canine

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

8C12B2

Cat no : 67791-1-Ig

Synonyms

AIFM1, PDCD8, EC:1.6.99.-, Apoptosis-inducing factor 1, mitochondrial

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Validation Data Gallery

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  WB analysis using 67791-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67791-1-Ig (AIF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67791-1-Ig

  WB result of AIF antibody (67791-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AIF transfected HeLa cells.

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  IHC staining of human kidney using 67791-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67791-1-Ig (AIF antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 67791-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67791-1-Ig (AIF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human kidney using 67791-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human kidney using 67791-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human kidney using 67791-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 67791-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 67791-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HCT 116 using 67791-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH-3T3 cells, MDCK cells, Pig brain cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human kidney tissue
• Positive IF/ICC detected in: HeLa cells, HCT 116 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

67791-1-Ig targets AIF in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, canine samples.

• Tested Reactivity: human, mouse, rat, pig, canine
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: AIF fusion protein Ag12400
• Full Name: apoptosis-inducing factor, mitochondrion-associated, 1
• Calculated Molecular Weight: 609 aa, 66 kDa
• Observed Molecular Weight: 67 kDa, 57 kDa
• GenBank Accession Number: BC111065
• Gene Symbol: AIF
• Gene ID (NCBI): 9131
• RRID: AB_2918555
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).

Publications

Species	Application	Title
human	WB	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu
rat	WB	Inflammation; Chlorogenic Acid Alleviates Hepatic Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Mitochondria-Mediated Apoptosis In Vivo and In Vitro; Authors - Kai Li
rat	IF	Adv Sci (Weinh); Use of Deep-Learning Assisted Assessment of Cardiac Parameters in Zebrafish to Discover Cyanidin Chloride as a Novel Keap1 Inhibitor Against Doxorubicin-Induced Cardiotoxicity; Authors - Changtong Liu
rat	WB	Front Pharmacol; Apigenin protects against ischemic stroke by increasing DNA repair; Authors - Niu Ping