CoraLite® Plus 488-conjugated AGXT Recombinant antibody
AGXT Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240305E7
Cat no : CL488-83385-6
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of CL488-83385-6 is AGXT Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | AGXT fusion protein Ag18062 |
Full Name | alanine-glyoxylate aminotransferase |
Calculated Molecular Weight | 392 aa, 43 kDa |
Observed Molecular Weight | 43 kDa |
GenBank Accession Number | BC132819 |
Gene Symbol | AGXT |
Gene ID (NCBI) | 189 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 AGXT antibody CL488-83385-6 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |