AGXT Recombinant antibody

AGXT Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240305E7

Cat no : 83385-6-RR

Synonyms

AGT, AGT1, AGXT, AGXT1, PH1, SPAT, SPT, TLH6

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Validation Data Gallery

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  WB analysis using 83385-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83385-6-RR (AGXT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83385-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83385-6-RR (AGXT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 83385-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AGXT antibody (83385-6-RR, Clone: 240305E7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity experiments of 83385-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83385-6-RR against Human AGXT were performed. The affinity constant is 0.226 nM.

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, HuH-7 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83385-6-RR is AGXT Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AGXT fusion protein Ag18062
• Full Name: alanine-glyoxylate aminotransferase
• Calculated Molecular Weight: 392 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC132819
• Gene Symbol: AGXT
• Gene ID (NCBI): 189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure.