AGXT Recombinant antibody, PBS Only

AGXT Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240305E7

Cat no : 83385-6-PBS

Synonyms

EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1, AGT

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Validation Data Gallery

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  WB analysis using 83385-6-RR (same clone as 83385-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83385-6-RR (AGXT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

• 

  WB analysis using 83385-6-RR (same clone as 83385-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83385-6-RR (AGXT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83385-6-RR (same clone as 83385-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AGXT antibody (83385-6-RR, Clone: 240305E7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83385-6-RR (same clone as 83385-6-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug AGXT Recombinant antibody (83385-6-RR, Clone:240305E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83385-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83385-6-RR against Human AGXT were performed. The affinity constant is 0.226 nM.

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Product Information

The immunogen of 83385-6-PBS is AGXT Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AGXT fusion protein Ag18062
• Full Name: alanine-glyoxylate aminotransferase
• Calculated Molecular Weight: 392 aa, 43 kDa
• GenBank Accession Number: BC132819
• Gene Symbol: AGXT
• Gene ID (NCBI): 189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.