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AGXT Recombinant antibody, PBS Only

AGXT Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240305E7

Cat no : 83385-6-PBS

Synonyms

AGT, AGT1, AGXT, AGXT1, PH1, SPAT, SPT, TLH6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGXT Recombinant antibody (83385-6-RR)

    WB analysis using 83385-6-RR (same clone as 83385-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83385-6-RR (AGXT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AGXT Recombinant antibody (83385-6-RR)

    WB analysis using 83385-6-RR (same clone as 83385-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83385-6-RR (AGXT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AGXT Recombinant antibody (83385-6-RR)

    IF Staining of HeLa using 83385-6-RR (same clone as 83385-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AGXT antibody (83385-6-RR, Clone: 240305E7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

  • Affinity experiments of 83385-6-RR

    Affinity experiments of 83385-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83385-6-RR against Human AGXT were performed. The affinity constant is 0.226 nM.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83385-6-PBS is AGXT Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AGXT fusion protein Ag18062
Full Name alanine-glyoxylate aminotransferase
Calculated Molecular Weight 392 aa, 43 kDa
GenBank Accession NumberBC132819
Gene Symbol AGXT
Gene ID (NCBI) 189
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.