AGXT Recombinant antibody
AGXT Recombinant Antibody for WB, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
240305E7
Cat no : 83385-6-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, L02 cells, HuH-7 cells |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83385-6-RR is AGXT Fusion Protein expressed in E. coli.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | AGXT fusion protein Ag18062 |
Full Name | alanine-glyoxylate aminotransferase |
Calculated Molecular Weight | 392 aa, 43 kDa |
Observed Molecular Weight | 43 kDa |
GenBank Accession Number | BC132819 |
Gene Symbol | AGXT |
Gene ID (NCBI) | 189 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure.
Protocols
Product Specific Protocols | |
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WB protocol for AGXT antibody 83385-6-RR | Download protocol |
IF protocol for AGXT antibody 83385-6-RR | Download protocol |
FC protocol for AGXT antibody 83385-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |