AGR2 Monoclonal antibody, PBS Only

AGR2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A8A8

Cat no : 66768-1-PBS

Synonyms

GOB 4, AGR 2, AG-2, AG2, AG 2

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Validation Data Gallery

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  WB analysis of pig stomach using 66768-1-Ig (same clone as 66768-1-PBS)

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 66768-1-Ig (same clone as 66768-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66768-1-Ig (same clone as 66768-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66768-1-Ig (AGR2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66768-1-Ig (same clone as 66768-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66768-1-Ig (AGR2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66768-1-Ig (same clone as 66768-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66768-1-Ig (same clone as 66768-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  IF Staining of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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  FC experiment of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

  1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human AGR2 (66768-1-Ig, Clone:1A8A8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

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Product Information

66768-1-PBS targets AGR2 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: AGR2 fusion protein Ag2919
• Full Name: anterior gradient homolog 2 (Xenopus laevis)
• Calculated Molecular Weight: 175 aa, 20 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC015503
• Gene Symbol: AGR2
• Gene ID (NCBI): 10551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used to aide in assessment of high-risk patients undergoing endoscopic procedures.