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AGR2 Monoclonal antibody

AGR2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A8A8

Cat no : 66768-1-Ig

Synonyms

GOB 4, AGR 2, AG-2, AG2, AG 2

Validation Data Gallery

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  • Western Blot (WB) analysis of pig stomach tissue using AGR2 Monoclonal antibody (66768-1-Ig)

    WB analysis of pig stomach using 66768-1-Ig

    pig stomach tissue were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using AGR2 Monoclonal antibody (66768-1-Ig)

    WB analysis of T-47D using 66768-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-29 cells using AGR2 Monoclonal antibody (66768-1-Ig)

    WB analysis of HT-29 using 66768-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AGR2 Monoclonal antibody (66768-1-Ig)

    IHC staining of human breast cancer using 66768-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66768-1-Ig (AGR2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AGR2 Monoclonal antibody (66768-1-Ig)

    IHC staining of human breast cancer using 66768-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66768-1-Ig (AGR2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using AGR2 Monoclonal antibody (66768-1-Ig)

    IF Staining of human colon cancer using 66768-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using AGR2 Monoclonal antibody (66768-1-Ig)

    IF Staining of human colon cancer using 66768-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using AGR2 Monoclonal antibody (66768-1-Ig)

    IF Staining of HT-29 using 66768-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HT-29 cells using AGR2 Monoclonal antibody (66768-1-Ig)

    FC experiment of HT-29 using 66768-1-Ig

    1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human AGR2 (66768-1-Ig, Clone:1A8A8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

Tested Applications

Positive WB detected inpig stomach tissue, T-47D cells, HT-29 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue
Positive IF/ICC detected inHT-29 cells, human colon cancer tissue
Positive FC (Intra) detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66768-1-Ig targets AGR2 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen AGR2 fusion protein Ag2919
Full Name anterior gradient homolog 2 (Xenopus laevis)
Calculated Molecular Weight 175 aa, 20 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC015503
Gene Symbol AGR2
Gene ID (NCBI) 10551
RRIDAB_2882114
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used to aide in assessment of high-risk patients undergoing endoscopic procedures.

Protocols

Product Specific Protocols
WB protocol for AGR2 antibody 66768-1-IgDownload protocol
IHC protocol for AGR2 antibody 66768-1-IgDownload protocol
IF protocol for AGR2 antibody 66768-1-IgDownload protocol
FC protocol for AGR2 antibody 66768-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cancer

LINC02273 drives breast cancer metastasis by epigenetically increasing AGR2 transcription.

Authors - Bingqiu Xiu
View Article
humanWB

Front Oncol

QKI-6 Suppresses Cell Proliferation, Migration, and EMT in Non-Small Cell Lung Cancer.

Authors - Haihua Zhang
View Article