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Tested Applications
| Positive WB detected in | HepG2 cells, HuH-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-81083 targets AFP in WB applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | AFP fusion protein Ag6089 Predict reactive species |
| Full Name | alpha-fetoprotein |
| Calculated Molecular Weight | 69 kDa |
| Observed Molecular Weight | 68-72 kDa |
| GenBank Accession Number | BC027881 |
| Gene Symbol | AFP |
| Gene ID (NCBI) | 174 |
| RRID | AB_3673723 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02771 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult (PubMed:24120489). AFP is considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker (PubMed: 18669658).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 AFP antibody CL750-81083 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
