AFM Monoclonal antibody, PBS Only

AFM Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G9F6

Cat no : 66113-1-PBS

Synonyms

afamin, afamin/AFM, AFM, ALB2, ALBA, ALF, Alpha Alb, Alpha albumin

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Validation Data Gallery

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  WB analysis of human plasma using 66113-1-Ig (same clone as 66113-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66113-1-Ig (AFM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

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  IP experiment of human plasma using 66113-1-Ig (same clone as 66113-1-PBS)

  IP result of anti-AFM (IP:66113-1-Ig, 5ug; Detection:66113-1-Ig 1:500) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66113-1-Ig (same clone as 66113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66113-1-Ig (AFM Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66113-1-Ig (same clone as 66113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66113-1-Ig (AFM Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66113-1-Ig (same clone as 66113-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AFM antibody (66113-1-Ig, Clone: 2G9F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66113-1-Ig (same clone as 66113-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AFM antibody (66113-1-Ig, Clone: 2G9F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66113-1-PBS targets AFM in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AFM fusion protein Ag16866
• Full Name: afamin
• Calculated Molecular Weight: 599 aa, 69 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC109020
• Gene Symbol: AFM
• Gene ID (NCBI): 173
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.