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AFM Monoclonal antibody

AFM Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2G9F6

Cat no : 66113-1-Ig

Synonyms

ALF, ALBA, ALB2, afamin/AFM, afamin

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using AFM Monoclonal antibody (66113-1-Ig)

    WB analysis of human plasma using 66113-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66113-1-Ig (AFM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human plasma using AFM Monoclonal antibody (66113-1-Ig)

    IP experiment of human plasma using 66113-1-Ig

    IP result of anti-AFM (IP:66113-1-Ig, 5ug; Detection:66113-1-Ig 1:500) with human plasma lysate 4000ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AFM Monoclonal antibody (66113-1-Ig)

    IHC staining of mouse brain using 66113-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66113-1-Ig (AFM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AFM Monoclonal antibody (66113-1-Ig)

    IHC staining of mouse brain using 66113-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66113-1-Ig (AFM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using AFM Monoclonal antibody (66113-1-Ig)

    IHC staining of human liver using 66113-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66113-1-Ig (AFM Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver tissue using AFM Monoclonal antibody (66113-1-Ig)

    IHC staining of human liver using 66113-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66113-1-Ig (AFM Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AFM Monoclonal antibody (66113-1-Ig)

    IF Staining of HeLa using 66113-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AFM antibody (66113-1-Ig, Clone: 2G9F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inhuman plasma
Positive IP detected inhuman plasma tissue
Positive IHC detected inmouse brain tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66113-1-Ig targets AFM in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AFM fusion protein Ag16866
Full Name afamin
Calculated Molecular Weight 599 aa, 69 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC109020
Gene Symbol AFM
Gene ID (NCBI) 173
RRIDAB_2881512
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.

Protocols

Product Specific Protocols
WB protocol for AFM antibody 66113-1-IgDownload protocol
IHC protocol for AFM antibody 66113-1-IgDownload protocol
IF protocol for AFM antibody 66113-1-IgDownload protocol
IP protocol for AFM antibody 66113-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

J Ginseng Res

A proteomic approach reveals the differential protein expression in Drosophila melanogaster treated with red ginseng extract (Panax ginseng)

Authors - Qing-Xiu Liu
View Article