AEG-1/MTDH Monoclonal antibody, PBS Only

• 

  WB analysis using 68592-1-Ig (same clone as 68592-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68592-1-Ig (AEG-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 68592-1-Ig (same clone as 68592-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 68592-1-Ig (same clone as 68592-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using AEG-1/MTDH antibody (68592-1-Ig, Clone: 1D8C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

AEG-1/MTDH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 68592-1-PBS

Clone No.1D8C2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AEG-1, MTDH, 1D8C2, 3D3/LYRIC, AEG 1

---

Product Information

68592-1-PBS targets AEG-1/MTDH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AEG-1/MTDH fusion protein Ag5033
• Full Name: metadherin
• Calculated Molecular Weight: 582 aa, 64 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC045642
• Gene Symbol: AEG-1/MTDH
• Gene ID (NCBI): 92140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q86UE4
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression.