Filter: AllWBIHCIF/ICC

WB analysis using 68592-1-Ig (same clone as 68592-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68592-1-Ig (AEG-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.
IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.
IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.
IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 68592-1-Ig (same clone as 68592-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 68592-1-Ig (same clone as 68592-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed A431 cells using AEG-1/MTDH antibody (68592-1-Ig, Clone: 1D8C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

AEG-1/MTDH Monoclonal antibody, PBS Only

AEG-1/MTDH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 68592-1-PBS

Clone No.1D8C2

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AEG-1, MTDH, 1D8C2, 3D3/LYRIC, AEG 1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

68592-1-PBS targets AEG-1/MTDH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag5033 Product name: Recombinant human AEG-1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 233-582 aa of BC045642 Sequence: EQLTTASFPVGSKKNKGDSHLNVQVSNFKSGKGDSTLQVSSGLNENLTVNGGGWNEKSVKLSSQISAGEEKWNSVSPASAGKRKAEPSAWSQDTGDANTNGKDWGRSWSDRSIFSGIGSTAEPVSQSTTSDYQWDVSRNQPYIDDEWSGLNGLSSADPNSDWNAPAEEWGNWVDEERASLLKSQEPIPDDQKVSDDDKEKGEGALPTGKSKKKKKKKKKQGEDNSTAQDTEELEKEIREDLPVNTSKTRPKQEKAFSLKTISTSDPAEVLVKNSQPIKTLPPATSTEPSVILSKSDSDKSSSQVPPILQETDKSKSNTKQNSVPPSQTKSETSWESPKQIKKKKKARRET Predict reactive species
Full Name	metadherin
Calculated Molecular Weight	582 aa, 64 kDa
Observed Molecular Weight	70-80 kDa
GenBank Accession Number	BC045642
Gene Symbol	AEG-1/MTDH
Gene ID (NCBI)	92140
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q86UE4
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression.