AEG-1/MTDH Monoclonal antibody

AEG-1/MTDH Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68592-1-Ig

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

AEG-1, MTDH, Astrocyte elevated gene-1 protein, AEG1, AEG 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inA431 cells, MCF-10A cells, A549 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells
Positive IHC detected inhuman rectal cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68592-1-Ig targets AEG-1/MTDH in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AEG-1/MTDH fusion protein Ag5033 Predict reactive species
Full Name metadherin
Calculated Molecular Weight 582 aa, 64 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC045642
Gene Symbol AEG-1/MTDH
Gene ID (NCBI) 92140
RRIDAB_3085291
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86UE4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression.

Protocols

Product Specific Protocols
WB protocol for AEG-1/MTDH antibody 68592-1-IgDownload protocol
IHC protocol for AEG-1/MTDH antibody 68592-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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