• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

AEG-1/MTDH Monoclonal antibody

AEG-1/MTDH Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D8C2

Cat no : 68592-1-Ig

Synonyms

3D3, 3D3/LYRIC, AEG 1, AEG1, LYRIC, LYRIC/3D3, metadherin, Metastasis adhesion protein, MTDH, Protein LYRIC

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using AEG-1/MTDH Monoclonal antibody (68592-1-Ig)

    WB analysis using 68592-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68592-1-Ig (AEG-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA431 cells, MCF-10A cells, A549 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68592-1-Ig targets AEG-1/MTDH in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AEG-1/MTDH fusion protein Ag5033
Full Name metadherin
Calculated Molecular Weight 582 aa, 64 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC045642
Gene Symbol AEG1/MTDH
Gene ID (NCBI) 92140
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression.

Protocols

Product Specific Protocols
WB protocol for AEG-1/MTDH antibody 68592-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols