CoraLite®594-conjugated Perilipin-2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Perilipin-2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

Cat no : CL594-15294

Synonyms

ADFP, Perilipin 2, ADRP/Perilipin 2, ADRP, Adipose differentiation-related protein

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Validation Data Gallery

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  IF Staining of HeLa using CL594-15294

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using CoraLite®594 Perilipin-2 antibody (CL594-15294) at dilution of 1:200.

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  IF Staining of HeLa using CL594-15294

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using CoraLite®594 ADRP/Perilipin 2 antibody (CL594-15294) at dilution of 1:400, CL488-Phalloidine (green).

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Tested Applications

• Positive IF/ICC detected in: oleic acid treated HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-15294 targets Perilipin-2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Perilipin-2 fusion protein Ag7539
• Full Name: adipose differentiation-related protein
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC005127
• Gene Symbol: Perilipin-2
• Gene ID (NCBI): 123
• RRID: AB_2919820
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

Publications

Species	Application	Title
	IF	bioRxiv; Cold induces brain region-selective neuronal activity-dependent lipid metabolism; Authors - Hyeonyoung Min