CoraLite® Plus 488-conjugated ACTN2 Polyclonal antibody

ACTN2 Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14221

Synonyms

Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, Alpha actinin 2, ACTN 2, actinin, alpha 2

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Validation Data Gallery

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  IF Staining of mouse heart using CL488-14221

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite® Plus 488 ACTN2 antibody (CL488-14221) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of H9C2 using CL488-14221

  Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using CoraLite® Plus 488 ACTN2 antibody (CL488-14221) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: mouse heart tissue
• Positive IF/ICC detected in: H9C2 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14221 targets ACTN2 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACTN2 fusion protein Ag5459
• Full Name: actinin, alpha 2
• Calculated Molecular Weight: 104 kDa
• Observed Molecular Weight: 103 kDa
• GenBank Accession Number: BC051770
• Gene Symbol: ACTN2
• Gene ID (NCBI): 88
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).