Validation Data Gallery
|Positive WB detected in||mouse brain tissue, C2C12 cells, mouse lung tissue, mouse skeletal muscle tissue, mouse heart, mouse kidney, rat skeletal muscle|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||mouse heart tissue, mouse skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Human iPSC derived cardiomyocyte, NIH/3T3 cells, C2C12 cells, mouse heart tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
14221-1-AP targets ACTN2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACTN2 fusion protein Ag5459|
|Full Name||actinin, alpha 2|
|Calculated molecular weight||104 kDa|
|Observed molecular weight||103 kDa|
|GenBank accession number||BC051770|
|Gene ID (NCBI)||88|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
|Product Specific Protocols|
|WB protocol for ACTN2 antibody 14221-1-AP||Download protocol|
|IHC protocol for ACTN2 antibody 14221-1-AP||Download protocol|
|IF protocol for ACTN2 antibody 14221-1-AP||Download protocol|
|IP protocol for ACTN2 antibody 14221-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Cell Biochem
Possible mechanism of GATA4 inhibiting myocardin activity during cardiac hypertrophy.
Biochem Biophys Res Commun
MiR-466h-5p induces expression of myocardin with complementary promoter sequences.
Dokl Biochem Biophys
Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?
Identification of differential microRNAs and messenger RNAs resulting from ASXL transcriptional regulator 3 knockdown during during heart development.
Prog Biophys Mol Biol
Biomimetic electrical stimulation induces rat bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells via TGF-beta 1 in vitro.
A ceRNA-associated risk model predicts the poor prognosis for head and neck squamous cell carcinoma patients.