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CoraLite®594-conjugated ACTC1-specific Monoclonal antibody

ACTC1-specific Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F2B9

Cat no : CL594-66125

Synonyms

ACTC1, Alpha actin, actin, ACTC1 cardiac muscle 1, ACTC 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite®594-conjugated ACTC1-specific Monoclonal (CL594-66125)

    IF Staining of mouse heart using CL594-66125

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CL594-66125 (ACTC1-specific antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite®594-conjugated ACTC1-specific Monoclonal (CL594-66125)

    IF Staining of mouse heart using CL594-66125

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CL594-66125 (ACTC1-specific antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of C2C12 cells using CoraLite®594-conjugated ACTC1-specific Monoclonal (CL594-66125)

    FC experiment of C2C12 using CL594-66125

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ACTC1-specific (CL594-66125, Clone:1F2B9) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF-P detected inmouse heart tissue
Positive FC (Intra) detected inC2C12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66125 targets ACTC1-specific in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name actin, alpha, cardiac muscle 1
Calculated Molecular Weight 42 kDa
GenBank Accession NumberNM_005159
Gene Symbol ACTC1
Gene ID (NCBI) 70
RRIDAB_2883519
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocols

Product Specific Protocols
IF protocol for CL594 ACTC1-specific antibody CL594-66125Download protocol
FC protocol for CL594 ACTC1-specific antibody CL594-66125Download protocol
Standard Protocols
Click here to view our Standard Protocols