ACTC1-specific Monoclonal antibody

ACTC1-specific Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1F2B9

Cat no : 66125-1-Ig

Synonyms

ACTC, ACTC1, ACTC1 cardiac muscle 1, ACTC1 specific, ACTC1-specific, Actin cardiac muscle specific, Actin, alpha cardiac muscle 1, actin, alpha, cardiac muscle 1, Alpha cardiac actin, CMD1R, CMH11



Tested Applications

Positive WB detected inhuman heart tissue, pig heart tissue, rat heart tissue, mouse heart tissue
Positive IHC detected inhuman heart tissue, human heart and human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue
Positive FC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:10000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66125-1-Ig targets ACTC1-specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name actin, alpha, cardiac muscle 1
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberNM_005159
Gene symbol ACTC1
Gene ID (NCBI) 70
RRIDAB_2881524
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.

Protocols

Product Specific Protocols
WB protocol for ACTC1-specific antibody 66125-1-IgDownload protocol
IHC protocol for ACTC1-specific antibody 66125-1-IgDownload protocol
IF protocol for ACTC1-specific antibody 66125-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Life Sci

Cadmium exposure induces a sex-dependent decline in left ventricular cardiac function

Authors - Michael L Fitch
WB

J Am Heart Assoc

Postinfarction Hearts Are Protected by Premature Senescent Cardiomyocytes Via GATA 4-Dependent CCN 1 Secretion.

Authors - Sumei Cui
humanIHC

Front Mol Biosci

Co-Overexpression of GRK5/ACTC1 Correlates With the Clinical Parameters and Poor Prognosis of Epithelial Ovarian Cancer.

Authors - Longyang Liu
humanWB

Immunol Res

The influences of α-hemolytic Streptococcus on class switching and complement activation of human tonsillar cells in IgA nephropathy.

Authors - Muyao Ye
mouseIF

Development

BMP4 triggers regulatory circuits specifying the cardiac mesoderm lineage

Authors - Pavel Tsaytler
ratWB,IF

Eur J Pharmacol

Puerarin ameliorates myocardial remodeling of spontaneously hypertensive rats through inhibiting TRPC6-CaN-NFATc3 pathway

Authors - Jiang Yan