ACTC1-specific Monoclonal antibody
ACTC1-specific Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 66125-1-Ig
Validation Data Gallery
|Positive WB detected in
|human heart tissue, pig heart tissue, rat heart tissue, mouse heart tissue
|Positive IHC detected in
|human heart tissue, human heart and human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|mouse heart tissue
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2500-1:10000
|IHC : 1:100-1:400
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66125-1-Ig targets ACTC1-specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
|human, mouse, pig, rat
|human, mouse, rat, rabbit
|Host / Isotype
|Mouse / IgG1
|actin, alpha, cardiac muscle 1
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.
Cadmium exposure induces a sex-dependent decline in left ventricular cardiac function
J Am Heart Assoc
Postinfarction Hearts Are Protected by Premature Senescent Cardiomyocytes Via GATA 4-Dependent CCN 1 Secretion.
Front Mol Biosci
Co-Overexpression of GRK5/ACTC1 Correlates With the Clinical Parameters and Poor Prognosis of Epithelial Ovarian Cancer.
The influences of α-hemolytic Streptococcus on class switching and complement activation of human tonsillar cells in IgA nephropathy.
BMP4 triggers regulatory circuits specifying the cardiac mesoderm lineage
Eur J Pharmacol
Puerarin ameliorates myocardial remodeling of spontaneously hypertensive rats through inhibiting TRPC6-CaN-NFATc3 pathway