Various lysates were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
WB analysis of HEK-293 using 67509-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 67509-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of pig brain using 67509-1-Ig
pig brain tissue were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
pig brain tissue were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 67509-1-Ig
rat brain tissue were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
rat brain tissue were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 67509-1-Ig
mouse brain tissue were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 67509-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67509-1-Ig (ACO2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67509-1-Ig (ACO2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67509-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67509-1-Ig (ACO2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67509-1-Ig (ACO2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HeLa using 67509-1-Ig
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ACO2 (67509-1-Ig, Clone:1F1G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ACO2 (67509-1-Ig, Clone:1F1G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
67509-1-Ig targets Aconitase 2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
ACO2(aconitate hydratase, mitochondrial) is also named as citrate hydro-lyase and belongs to the aconitase/IPM isomerase family. It plays a key function in cellular energy production, and loss of its activity has a major impact on cellular and organismal survival. Western blot shows two bands of 83 kDa and 40 kDa. The 40 kDa fragment decreases with age and oxidative stress, whereas other fragmentation products with molecular weights between 40 and 83 kDa increased with age and MnSOD(mitochondrial manganese superoxide dismutase) deficiency(PMID:12459471). Defects in ACO2 are the cause of infantile cerebellar-retinal degeneration (ICRD).
Various lysates were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
WB analysis of HEK-293 using 67509-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 67509-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of pig brain using 67509-1-Ig
pig brain tissue were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 67509-1-Ig
rat brain tissue were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 67509-1-Ig
mouse brain tissue were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver cancer using 67509-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67509-1-Ig (ACO2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67509-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67509-1-Ig (ACO2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC (INTRA) Figures
FC experiment of HeLa using 67509-1-Ig
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ACO2 (67509-1-Ig, Clone:1F1G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Aconitase 2 Monoclonal antibody
Catalog Number
67509-1-Ig
Citations
-
Dilutions
WB : 1:5000-1:50000 IHC : 1:500-1:2000 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, FC (Intra), ELISA
Reactivity
human, mouse, rat, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
