ACO2 Monoclonal antibody

ACO2 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1F1G4

Cat no : 67509-1-Ig

Synonyms

aconitase 2, Citrate hydro-lyase, Aconitase2, Aconitase, ACO 2

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Validation Data Gallery

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  WB analysis using 67509-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HEK-293 using 67509-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67509-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67509-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67509-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67509-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67509-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67509-1-Ig (ACO2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67509-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67509-1-Ig (ACO2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 67509-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ACO2 (67509-1-Ig, Clone:1F1G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: PC-12 cells, pig brain tissue, rat brain tissue, mouse brain tissue, HEK-293 cells, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue, Neuro-2a cells, rabbit brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67509-1-Ig targets ACO2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACO2 fusion protein Ag17784
• Full Name: aconitase 2, mitochondrial
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC014092
• Gene Symbol: ACO2
• Gene ID (NCBI): 50
• RRID: AB_2882730
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACO2(aconitate hydratase, mitochondrial) is also named as citrate hydro-lyase and belongs to the aconitase/IPM isomerase family. It plays a key function in cellular energy production, and loss of its activity has a major impact on cellular and organismal survival. Western blot shows two bands of 83 kDa and 40 kDa. The 40 kDa fragment decreases with age and oxidative stress, whereas other fragmentation products with molecular weights between 40 and 83 kDa increased with age and MnSOD(mitochondrial manganese superoxide dismutase) deficiency(PMID:12459471). Defects in ACO2 are the cause of infantile cerebellar-retinal degeneration (ICRD).