14-3-3 Sigma Polyclonal antibody, PBS Only

14-3-3 Sigma Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10622-1-PBS

Synonyms

SFN, HME1, 14-3-3sigma, 14-3-3 protein sigma

---

Validation Data Gallery

• 

  WB analysis using 10622-1-AP (same clone as 10622-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 10622-1-AP (same clone as 10622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 10622-1-AP (same clone as 10622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bowen disease using 10622-1-AP (same clone as 10622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10622-1-AP (14-3-3 Sigma antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bowen disease using 10622-1-AP (same clone as 10622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10622-1-AP (14-3-3 Sigma antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

---

Product Information

10622-1-PBS targets 14-3-3 Sigma in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: 14-3-3 Sigma fusion protein Ag0973
• Full Name: stratifin
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC002995
• Gene Symbol: 14-3-3 Sigma
• Gene ID (NCBI): 2810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.