14-3-3 Sigma Polyclonal antibody

14-3-3 Sigma Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10622-1-AP

Synonyms

SFN, HME1, 14-3-3sigma, 14-3-3 protein sigma

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Validation Data Gallery

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  WB analysis using 10622-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skin using 10622-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:500 (under 40x lens).

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  IHC staining of mouse skin using 10622-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human bowen disease using 10622-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10622-1-AP (14-3-3 Sigma antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 10622-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10622-1-AP (14-3-3 Sigma antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, A549 cells, HeLa cells, U2OS cells
• Positive IHC detected in: mouse skin tissue, human bowen disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

10622-1-AP targets 14-3-3 Sigma in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: 14-3-3 Sigma fusion protein Ag0973
• Full Name: stratifin
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC002995
• Gene Symbol: 14-3-3 Sigma
• Gene ID (NCBI): 2810
• RRID: AB_2254479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.

Publications

Species	Application	Title
human	WB	Sci Rep; Sting and p53 DNA repair pathways are compromised in Alzheimer's disease; Authors - Thomas J Nelson
human	WB	Phytomedicine; Acetylation of MOB1 mediates polyphyllin II-reduced lysosome biogenesis in breast cancer by promoting the cytoplasmic retention of the YAP/TFEB coactivator complex; Authors - Yuliang Ren
human,mouse	WB,IHC	J Proteome Res; Quantitative Proteomics Reveal the Mechanism of MiR-138-5p Suppressing Cervical Cancer via Targeting ZNF385A; Authors - Qihang Peng