CoraLite® Plus 488-conjugated 14-3-3 Monoclonal antibody

14-3-3 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

CloneNo.

2E10A3

Cat no : CL488-66061

Synonyms

14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 1C5, HS1, Protein HS1, YWHAQ

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-66061

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66061 (14-3-3 antibody) at dilution of 1:50.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of CL488-66061 is 14-3-3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat, mouse, pig
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: 14-3-3 fusion protein Ag6055
• Full Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 31 kDa, 28 kDa
• GenBank Accession Number: BC056867
• Gene Symbol: 14-3-3 theta
• Gene ID (NCBI): 10971
• RRID: AB_2883245
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

14-3-3 proteins interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

Publications

Species	Application	Title
rat	IF	Front Pharmacol; Aspirin in combination with gastrodin protects cardiac function and mitigates gastric mucosal injury in response to myocardial ischemia/reperfusion; Authors - Zhiwu Dong