14-3-3 Monoclonal antibody, PBS Only

14-3-3 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E10A3

Cat no : 66061-1-PBS

Synonyms

14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3, 14-3-3 tau, HS1, Protein HS1, YWHAQ

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Validation Data Gallery

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  WB analysis using 66061-1-Ig (same clone as 66061-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66061-1-Ig (14-3-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 66061-1-Ig (same clone as 66061-1-PBS)

  IP result of anti-14-3-3 (IP:66061-1-Ig, 4ug; Detection:66061-1-Ig 1:20000) with rat brain tissue lysate 1080 ug. This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66061-1-Ig (same clone as 66061-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66061-1-Ig (same clone as 66061-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66061-1-Ig (same clone as 66061-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66061-1-Ig (same clone as 66061-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66061-1-Ig (same clone as 66061-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66061-1-Ig (14-3-3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66061-1-PBS targets 14-3-3 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, rat, mouse, pig samples.

• Tested Reactivity: human, rat, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: 14-3-3 fusion protein Ag6055
• Full Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 31 kDa, 28 kDa
• GenBank Accession Number: BC056867
• Gene Symbol: 14-3-3 theta
• Gene ID (NCBI): 10971
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

14-3-3 proteins interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.