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Neuropilin 1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat No : MP01310-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01310-4, Human Neuropilin 1 Recombinant Matched Antibody Pair - PBS only. 84429-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2331. 84429-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

    Sandwich ELISA standard curve of MP01310-4

    Sandwich ELISA standard curve of MP01310-4, Human Neuropilin 1 Recombinant Matched Antibody Pair - PBS only. 84429-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2331. 84429-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Serum of six individual healthy human donors was measured. The Neuropilin 1 concentration of detected samples was determined to be 338.2 ng/mL with a range of 291.5-420.8 ng/mL.

    Sample test of MP01310-4

    Serum of six individual healthy human donors was measured. The Neuropilin 1 concentration of detected samples was determined to be 338.2 ng/mL with a range of 291.5-420.8 ng/mL.

  • Human milk of six individual healthy human donors was measured. The Neuropilin 1 concentration of detected samples was determined to be 69.2 ng/mL with a range of 34.6-102.0 ng/mL.

    Sample test of MP01310-4

    Human milk of six individual healthy human donors was measured. The Neuropilin 1 concentration of detected samples was determined to be 69.2 ng/mL with a range of 34.6-102.0 ng/mL.

  • The mean Neuropilin 1 concentration was determined to be 5.5 ng/mL in HT-29 cell extract based on a 1.5 mg/mL extract load.

    Sample test of MP01310-4

    The mean Neuropilin 1 concentration was determined to be 5.5 ng/mL in HT-29 cell extract based on a 1.5 mg/mL extract load.

  • HT-29 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human Neuropilin 1, and measured 3.1 ng/mL.

    Sample test of MP01310-4

    HT-29 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human Neuropilin 1, and measured 3.1 ng/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01310-4 targets Neuropilin 1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: NRP1 Recombinant antibody, PBS Only (Capture) 84429-2-PBS (241812B7). 100 μg. Concentration 1 mgl/ml.

Detection antibody: NRP1 Recombinant antibody, PBS Only (Capture/Detector) 84429-4-PBS (241812E11). 100 μg. Concentration 1 mgl/ml.

Matched pair protein standard: Eg2331

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84429-2-PBS
Tested Reactivity human
Immunogen FusionProtein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name neuropilin 1
Synonyms neuropilin 1, Neuropilin-1, CD304, BDCA4, 241812B7
GenBank accession numberBC007533
Gene symbol Neuropilin 1
UniprotO14786
Gene ID (NCBI) 8829
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84429-4-PBS
Tested Reactivity human
Immunogen FusionProtein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name neuropilin 1
Synonyms neuropilin 1, Neuropilin-1, CD304, BDCA4, 241812E11
GenBank accession numberBC007533
Gene symbol Neuropilin 1
UniprotO14786
Gene ID (NCBI) 8829
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.