Neuropilin 1 Recombinant antibody, PBS Only (Capture)

NRP1 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241812B7

Cat no : 84429-2-PBS

Synonyms

neuropilin 1, Neuropilin-1, CD304, BDCA4, 241812B7

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01310-4

  Sandwich ELISA standard curve of MP01310-4, Human Neuropilin 1 Recombinant Matched Antibody Pair - PBS only. 84429-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2331. 84429-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

• 

  Sample test of MP01310-4

  Human milk of six individual healthy human donors was measured. The Neuropilin 1 concentration of detected samples was determined to be 69.2 ng/mL with a range of 34.6-102.0 ng/mL.

• 

  Sample test of MP01310-4

  The mean Neuropilin 1 concentration was determined to be 5.5 ng/mL in HT-29 cell extract based on a 1.5 mg/mL extract load.

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  Sample test of MP01310-4

  HT-29 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human Neuropilin 1, and measured 3.1 ng/mL.

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  Sample test of MP01310-4

  Serum of six individual healthy human donors was measured. The Neuropilin 1 concentration of detected samples was determined to be 338.2 ng/mL with a range of 291.5-420.8 ng/mL.

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  Cytometric bead array standard curve of MP01310-3

  Cytometric bead array standard curve of MP01310-3, Neuropilin 1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84429-2-PBS. Detection antibody: 84429-1-PBS. Standard: Eg2331. Range: 0.781-100 ng/mL.

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Product Information

84429-2-PBS targets NRP1 as part of a matched antibody pair:

MP01310-3: 84429-2-PBS capture and 84429-1-PBS detection (validated in Cytometric bead array)

MP01310-4: 84429-2-PBS capture and 84429-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FusionProtein
• Full Name: neuropilin 1
• GenBank Accession Number: BC007533
• Gene Symbol: Neuropilin 1
• Gene ID (NCBI): 8829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.