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  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD14 (UCHM-1) (FITC-65056)

    FC experiment of human PBMCs using FITC-65056

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD14 (UCHM-1) (FITC-65056, Clone: UCHM-1) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD14 (UCHM-1) (FITC-65056)

    FC experiment of human PBMCs using FITC-65056

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD14 (UCHM-1) (FITC-65056, Clone: UCHM-1) (red) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

FITC Plus Anti-Human CD14 (UCHM-1)

CD14 Monoclonal Antibody for FC
Cat No. FITC-65056
Clone No.UCHM-1
Publications(1)

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

UCHM-1, Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  FITC Plus
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

FITC-65056 targets CD14 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Thymocytes and peripheral blood lymphocytes from a remission AML patient Predict reactive species
Full Name CD14 molecule
Calculated Molecular Weight 375 aa, 40 kDa
GenBank Accession NumberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
RRIDAB_2883746
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP08571
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
FC protocol for FITC Plus CD14 antibody FITC-65056Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

J Bone Miner Res

Single-cell RNA sequencing reveals that an imbalance in monocyte subsets rather than changes in gene expression patterns is a feature of postmenopausal osteoporosis

Authors - Lin Tao
View Article