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FITC Plus Anti-Human CD107b / LAMP2 (H4B4)

CD107b / LAMP2 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

H4B4

Cat no : FITC-65053

Synonyms

LAMP2, CD107b, H4B4, LAMP 2, CD107 antigen-like family member B, LAMP-2, LAMPB, LGP110, LGP-96, Lysosome-associated membrane glycoprotein 2, Lysosome-associated membrane protein 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FITC Plus Anti-Human CD107b / LAMP2 (H4B4) (FITC-65053)

    IF Staining of HeLa using FITC-65053

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FITC-65053 (CD107b antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD107b / LAMP2 (H4B4) (FITC-65053)

    FC experiment of human PBMCs using FITC-65053

    1X10^6 PHA-activated human PBMCs were surface stained with 5 ul FITC Plus Plus Anti-Human CD107b (FITC-65053, Clone: H4B4). Unactivated PBMCs were used as negative control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD107b / LAMP2 (H4B4) (FITC-65053)

    FC experiment of human PBMCs using FITC-65053

    1X10^6 PHA-activated human PBMCs were surface stained with 5 ul FITC Plus Plus Anti-Human CD107b (FITC-65053, Clone: H4B4). Unactivated PBMCs were used as negative control. Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65053 targets CD107b / LAMP2 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human adherent peripheral blood cells
Full Name lysosomal-associated membrane protein 2
Calculated Molecular Weight 45 kDa
GenBank Accession NumberBC002965
Gene Symbol LAMP2
Gene ID (NCBI) 3920
RRIDAB_2883743
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

Protocols

Product Specific Protocols
IF protocol for FITC Plus CD107b / LAMP2 antibody FITC-65053Download protocol
Standard Protocols
Click here to view our Standard Protocols