PE Anti-Human CD107b / LAMP2 (H4B4)
CD107b / LAMP2 Monoclonal Antibody for IF, FC (Intra)
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human
Applications
IF, FC (Intra)
Conjugate
PE Fluorescent Dye
CloneNo.
H4B4
Cat no : PE-65053
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HeLa cells |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
PE-65053 targets CD107b / LAMP2 in IF, FC (Intra) applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | Human adherent peripheral blood cells |
Full Name | lysosomal-associated membrane protein 2 |
Calculated Molecular Weight | 45 kDa |
GenBank Accession Number | BC002965 |
Gene Symbol | LAMP2 |
Gene ID (NCBI) | 3920 |
RRID | AB_2883850 |
Conjugate | PE Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.1% sodium azide and a stabilizer. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).
Protocols
Product Specific Protocols | |
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IF protocol for PE CD107b / LAMP2 antibody PE-65053 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |