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Atlantic Blue™ Anti-Human CD107b / LAMP2 (H4B4)

CD107b / LAMP2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC (Intra)

Conjugate

Atlantic Blue™ Fluorescent Dye

CloneNo.

H4B4

Cat no : AB-65053

Synonyms

CD107b, LAMP 2, LAMP2, LAMPB, LGP110

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using Atlantic Blue™ Anti-Human CD107b / LAMP2 (H4B4) (AB-65053)

    FC experiment of HeLa using AB-65053

    1X10^6 HeLa cells were intracellularly stained with 5 ul Atlantic Blue™ Anti-Human CD107b (AB-65053, Clone:H4B4). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

  • Flow cytometry (FC) experiment of HeLa cells using Atlantic Blue™ Anti-Human CD107b / LAMP2 (H4B4) (AB-65053)

    FC experiment of HeLa using AB-65053

    1X10^6 HeLa cells were intracellularly stained with 5 ul Atlantic Blue™ Anti-Human LAMP2 (AB-65053, Clone:H4B4). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

Tested Applications

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

AB-65053 targets CD107b / LAMP2 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human adherent peripheral blood cells
Full Name lysosomal-associated membrane protein 2
Calculated Molecular Weight 45 kDa
GenBank Accession NumberBC002965
Gene Symbol LAMP2
Gene ID (NCBI) 3920
Conjugate Atlantic Blue™ Fluorescent Dye
Excitation/Emission Maxima Wavelengths404 nm / 458 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).