Anti-Human CD45RO (UCHL1)

CD45RO Monoclonal Antibody for IF, FC

Host / Isotype

Mouse / IgG2a, kappa









Cat no : 65150-1-Ig


B220, CD45R, CD45RO, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200, UCHL1

Tested Applications

Positive IF detected inhuman colon cancer tissue
Positive FC detected inhuman PBMCs

Recommended dilution

Immunofluorescence (IF)IF : 1:200-1:800
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65150-1-Ig targets CD45RO in IF, FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen IL-2 dependent T cell line, CA1
Full Name protein tyrosine phosphatase, receptor type, C
GenBank Accession NumberBC014239
Gene Symbol PTPRC
Gene ID (NCBI) 5788
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).


Product Specific Protocols
IF protocol for CD45RO antibody 65150-1-IgDownload protocol
FC protocol for CD45RO antibody 65150-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols