CoraLite® Plus 647 Anti-Human CD45RO (UCHL1)

CD45RO Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

UCHL1

Cat no : CL647-65150

Synonyms

B220, CD45R, CD45RO, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200, UCHL1

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Validation Data Gallery

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  FC experiment of human peripheral blood lymphocytes using CL647-65150

  1X10^6 human peripheral blood lymphocytes were surface stained with FITC Anti-Human CD45RA (FITC-65108, Clone: HI100) and 5.00 ul CoraLite® Plus 647-conjugated Anti-Human CD45RO (CL647-65150, Clone: UCHL1). Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using CL647-65150

  1X10^6 human peripheral blood lymphocytes were surface stained with FITC Anti-Human CD45RA (FITC-65108, Clone: HI100) and CoraLite® Plus 647-conjugated Mouse IgG2a isotype control antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: human peripheral blood lymphocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65150 targets CD45RO in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-2 dependent T cell line, CA1
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC014239
• Gene Symbol: CD45RO
• Gene ID (NCBI): 5788
• RRID: AB_2883714
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).