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CoraLite® Plus 555 Rat IgG2b Isotype Control (LTF-2)

IgG2b Isotype Control Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

LTF-2

Cat no : CL555-65211

Synonyms

IgG2b, IgG2b Isotype Control, Rat IgG2b Isotype Control

Validation Data Gallery

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  • Flow cytometry (FC) experiment of BALB/c mouse splenocytes using CoraLite® Plus 555 Rat IgG2b Isotype Control (LTF- (CL555-65211)

    FC experiment of BALB/c mouse splenocytes using CL555-65211

    1X10^6 BALB/c mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 555 Rat IgG2b Isotype Control (CL555-65211, Clone: LTF-2) (red), or 0.5 ug CoraLite® Plus 555 Anti-Mouse CD4 (green), or unstained (blue). Cells were not fixed.

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-65211 targets IgG2b Isotype Control in FC applications and shows reactivity with samples.

Tested Reactivity
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name IgG2b Isotype Control
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodN/A
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The LTF-2 immunoglobulin is useful as an isotype-matched control. The LTF-2 immunolglobulin has an unknown binding specificity and is used as an isotype control for rat IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 IgG2b Isotype Control antibody CL555-65211Download protocol
Standard Protocols
Click here to view our Standard Protocols