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CoraLite® Plus 555 Mouse IgG2a Isotype Control (C1.18.4)

IgG2a Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

C1.18.4

Cat no : CL555-65208

Synonyms

IgG2a, IgG2a Isotype Control, Mouse IgG2a Isotype Control

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 555 Mouse IgG2a Isotype Control (C1 (CL555-65208)

    FC experiment of human PBMCs using CL555-65208

    1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 555 Anti-Mouse IgG2a Isotype Control (CL555-65208, Clone:C1.18.4) (red), or CL555 Anti-Human CD3 (OKT3) (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected in

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-65208 targets IgG2a Isotype Control in FC applications and shows reactivity with samples.

Tested Reactivity
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name IgG2a Isotype Control
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The C1.18.4 immunoglobulin is useful as an isotype-matched control. The C.1.18.4 immunolglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 IgG2a Isotype Control antibody CL555-65208Download protocol
Standard Protocols
Click here to view our Standard Protocols