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APC Anti-Mouse CD45R (B220) (RA3-6B2)

CD45R (B220) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

RA3-6B2

Cat no : APC-65139

Synonyms

Cd45, Ptprc, RA3-6B2, Leukocyte common antigen, LCA

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using APC Anti-Mouse CD45R (B220) (RA3-6B2) (APC-65139)

    FC experiment of mouse splenocytes using APC-65139

    1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 488 Anti-Mouse CD3 (17A2) and 0.1 ug APC Anti-Mouse CD45R (B220) (APC-65139, Clone:RA3-6B2) or 0.1 ug APC-Rat IgG2a isotype control. Cells were not fixed.

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65139 targets CD45R (B220) in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Abelson murine leukemia virus-induced pre-B tumor cells
Full Name protein tyrosine phosphatase, receptor type, C
GenBank Accession NumberBC028512
Gene Symbol CD45
Gene ID (NCBI) 19264
RRIDAB_2883020
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.

Protocols

Product Specific Protocols
FC protocol for APC CD45R (B220) antibody APC-65139Download protocol
Standard Protocols
Click here to view our Standard Protocols